Electrochemical Immunoassay by Capillary Electrophoresis forE. coliEnhanced by Field-ampli?ed Sample Injection and Gold Nanoparticles
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چکیده
منابع مشابه
Determination of ephedrine and pseudoephedrine by field-amplified sample injection capillary electrophoresis.
A simple and rapid capillary electrophoresis method was developed for the separation and determination of ephedrine (E) and pseudoephedrine (PE) in a buffer solution containing 80 mM of NaH2PO4 (pH 3.0), 15 mM of β-cyclodextrin and 0.3% of hydroxypropyl methylcellulose. The field-amplified sample injection (FASI) technique was applied to the online concentration of the alkaloids. With FASI in t...
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Due to high resolution power of sulfated cyclodextrins (HS-CDs), utilization of these selectors for chiral resolution of 7 basic and 2 zwitterionic drugs have been examined. Experiments were performed on a HP3DCE instrument equipped with an on-column diode array UV absorbance detector. Fused silica capillaries with an inner diameter of 50 ?m, an outer diameter of 365 ?m, and a total length of 4...
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Due to high resolution power of sulfated cyclodextrins (HS-CDs), utilization of these selectors for chiral resolution of 7 basic and 2 zwitterionic drugs have been examined. Experiments were performed on a HP3DCE instrument equipped with an on-column diode array UV absorbance detector. Fused silica capillaries with an inner diameter of 50 ?m, an outer diameter of 365 ?m, and a total length of 4...
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Here we show that a closed pneumatic microvalve on a PDMS chip can serve as a semipermeable membrane under an applied potential, enabling current to pass through while blocking the passage of charged analytes. Enrichment of both anionic and cationic species has been demonstrated, and concentration factors of ~70 have been achieved in just 8 s. Once analytes are concentrated, the valve is briefl...
متن کاملEnzyme immunoassay by capillary electrophoresis with laser induced fluorescence detection
Antibody (Ab) labeled with alkaline phosphatase was separated and detected by a capillary electrophoresis system equipped with a laser induced fluorescence detection system. The combination of B/F separation and the LIF detection of the label enzyme is potentially useful for a highly sensitive detection of antigen (Ag). After B/F separation by capillary electrophoresis (CE), label enzymes catal...
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ژورنال
عنوان ژورنال: Acta Chimica Sinica
سال: 2012
ISSN: 0567-7351
DOI: 10.6023/a12080567